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Reeve was born Dana Charles Morosini in Teaneck, New Jersey to Charles Morosini, a cardiologist, and Helen Simpson Morosini, who died in 2005.

She grew up in the town of Greenburgh, New York, where she graduated from Edgemont High School in 1979. She graduated cum laude in English Literature from Middlebury College in Vermont in 1984.

She spent the junior year of her studies at the Royal Academy of Dramatic Art in London. She later pursued additional graduate studies in acting at the California Institute of the Arts. She and her husband received honorary Doctorates of Humane Letters from Middlebury in 2004.

She married actor Christopher Reeve in Williamstown, Massachusetts in April 1992, and they had one child, William Elliot "Will" Reeve, born on June 7, 1992, whom they raised in Pound Ridge, New York.

On August 9, 2005 Reeve announced that, although she had never smoked cigarettes, she had been diagnosed with lung cancer. Reeve chose to disclose her illness after The National Enquirer announced that it planned to make the information public.

In 2005, Reeve received the "Mother of the Year Award" from the American Cancer Society for her dedication and determination in raising her son after the loss of her husband. In her final public appearances, Reeve stated that the tumor had responded to therapy and was shrinking. She appeared at Madison Square Garden on January 12, 2006, to sing in honor of New York Rangers hockey player, Mark Messier, whose number was retired that evening.

Reeve died on March 6, 2006, aged 44, at Memorial Sloan-Kettering Cancer Center in New York City. She is survived by her son, her father, two sisters, Deborah Morosini and Adrienne Morosini Heilman; and her late husband`s two grown children (her stepchildren), Matthew Exton Reeve and Alexandra Exton Reeve.