Anna Maria Martinozzi was born in Rome to Girolamo Martinozzi and Laura Margherita Mazzarini, the elder sister of Jules Mazarin, who was Cardinal and Prime Minister during the minority of Louis XIV.
She and her younger sister Laura were brought to France by her uncle, as were her maternal cousins, the Mancini sisters. The seven nieces of Cardinal Mazarin came to be known as the Mazarinettes by the French court. Mazarin managed to secure advantageous marriages for all of them.
In 1654, she married Armand de Bourbon, Prince of Conti (1629–1666). The marriage took place at the Palais du Louvre 22 February 1654; The couple had three children, two of whom reached adulthood:
- Louis de Bourbon (Hôtel de Conti, 6 September 1658 - Hôtel de Conti, 14 September 1658).
- Louis Armand de Bourbon, Prince of Conti (Hôtel de Conti, 4 April 1661- Palace of Fontainebleau 9 November 1685).
- François Louis de Bourbon, Prince of Conti (Hôtel de Conti, 30 April 1664 - Hôtel de Conti, 22 February 1709).
She acted as the godmother by proxy to le Grand Dauphin for Henrietta Maria of France, the dauphins own aunt (24 March 1668).
Anne Marie died in Paris at the Hôtel de Conti; she was aged roughly 35.