Francesco II Gonzaga, Marquess of Mantua and Isabella d'Este were married for 29 years before Francesco II Gonzaga, Marquess of Mantua died, leaving behind his partner and 8 children.

They had 8 children, Eleonora Gonzaga, Duchess of Urbino (532), Margherita (529), Federico II Gonzaga, Duke of Mantua (526), Livia (525), Ippolita Gonzaga (522), Ercole Gonzaga (521), Ferrante Gonzaga (519) and Paola Gonzaga (517).

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