They had a son named Charles.
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At the age of 16, l'Aubespine married Nicolas de Neufville, seigneur de Villeroy an already well-established, well-traveled minister of the king who would, over the course of his life, serve as secretary of state under four French kings from both the House of Valois and the House of Bourbon.
Villeroy and Madelaine had two sons and a daughter, but only one son, named Charles after the king, survived. He who would become the governor of Lyon and in 1619, made a marquis by Louis XIII.
Villeroy remarried after his wife's death in 1596 and had another son, Nicolas.